Shanghai Hengyuan Professional Agent ELISA Kit: Interleukin 6 Manual Download

Shanghai Hengyuan professional agent pig ELISA kit: Interleukin 6 manual download Experimental principle: This kit uses the double antibody sandwich method to determine the level of porcine interleukin 6 (IL-6) in the specimen. Microporous plates were coated with purified porcine interleukin 6 (IL-6) antibody to make solid-phase antibodies, and interleukin 6 (IL-6) was added to the microwells coated with mAb in turn, and then labeled with HRP The interleukin 6 (IL-6) antibody binds to form an antibody-antigen-enzyme-labeled antibody complex, and after thorough washing, the substrate TMB is used for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with interleukin 6 (IL-6) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of porcine interleukin 6 (IL-6) in the sample was calculated by a standard curve. Learn more about the operation steps, precautions, and composition of the porcine interleukin 6 ELISA kit. You are welcome to click to download. Shanghai Hengyuan thank you for your support!

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