Carbapenem antibiotics are one of the last antibiotics that can fight infections that are ineffective against many other drugs. However, pathogens resistant to carbapenems have been produced decades ago. To determine if a pathogen has an enzyme that removes carbapenems (carbonase), Chinese scientists have developed a simple and fast method based on fluorescent probes and optical detection, which they introduced in Angewandte Chemie. Their latest achievements.
Carbapenem antibiotics are a class of beta-lactam antibiotics similar to cephalosporins and penicillins. Although some bacterial strains can resist beta-lactam antibiotics by producing beta-lactamases, most beta-lactamases do not affect the function of carbapenems. Therefore, these antibiotics are also called the last antibiotics. They are effective drugs for treating several serious diseases, such as urinary tract infections caused by drug-resistant bacteria, abdominal infections, and pneumonia caused by hospitalization. But there is growing evidence that some bacteria can produce carbapenemase to cause resistance. Now, Xie Hexin and his team from East China University of Science and Technology have developed a new strategy to detect pathogens carrying carbapenemases.
The researchers synthesized a substance with the same structure as carbapenem, but bonded it with a fluorescent dye. If this carbapenem analog (CVB-1) is recognized by the carbapenemase in the bacterial extract, CVB-1 is cleaved and decomposed. When the electronic interaction of the dye with carbapenem disappears due to cleavage, the dye turns into a green fluorescent molecule, which means that if illuminated with a certain wavelength of light, these molecules will glow green. Therefore, the method of detection is as follows: If carbapenemase is present in the bacterial extract, the sample will emit green light at a specific wavelength after a period of time.
This technology allows us to detect bacterial antibiotic resistance by fluorescence. Therefore, using this fluorescence-based detection system, doctors can detect whether the infection is caused by carbapenem-resistant bacteria in a very short time. Based on this result, doctors can give more personalized treatment to prevent overuse of ineffective drugs. Scientists have conducted several tests to prove that their CVB-1 test is specific and has a low detection limit and can be used for in vivo detection. This simple and fast detection method based on fluorescence is an excellent means of detecting antibiotic-resistant bacteria that are becoming more and more serious.
Shanghai Chuangsai Technology has excellent performance, interleukin cytokines, fetal bovine serum, electrophoresis equipment scientific instruments, raw material drug standards, chemical reagents, cell culture consumables, Shanghai Chuangsai, mass products special promotions, welcome to inquire!
DUOBUY TECHNOLOGY SHANDONG CO., LTD. , https://www.doubuytech.com