Nowadays, due to the convenience of ELISA kit detection, many ordinary Baixin will also purchase ELISA kits for experiments. There are many kinds of ELISA kits, such as elisa kit, mouse elisa kit, rat elisa kit, etc. In people's minds, imported ELISA kits are still better than domestic ELISA kits. Whether it is imported or domestic ELISA kits, we must not ignore the background influence of the experiment during the experiment, so how can we minimize the impact of the test background? Start with the following aspects:
1. Washing: The seemingly boring step is actually the top priority of the experiment, because if unbound material (such as non-specifically bound antibodies or detection reagents) remains in the microplate, it will increase the background noise. If necessary, increase the salt concentration in the wash solution, which prevents non-specific binding reactions. If the background is too high and you suspect that the wash is not enough, try increasing the number of washes.
2. Sealing: The effect of the blocking solution is to allow unrelated proteins to occupy potential binding sites in the microplate. This reduces the chance of non-specific binding of antibodies that produce signals. Of course, you also want antibodies to bind only to the protein of interest. If your background is too high and you suspect that the closure is not adequate, you can try a higher concentration of blocking solution or extend the closure time appropriately.
If you have been plagued by background issues, then maybe you should spend some time optimizing the sealant. This may take time, but it is also worth it. There are currently two main types of blocking fluids: protein and non-ionic detergents. The type you use depends on several factors, including the surface reagents of the microplate, the antigen adsorbed by the ELISA kit, your antibodies, and the test reagents. A good blocking solution should reduce non-specific binding, but it should not interact with antigens, antibodies or detection reagents.
3. Antibody concentration: In the ELISA experiment, we usually follow the steps left by the brothers and sisters, but if the reagents are slightly different, it may be necessary to optimize the amount of antibody. Remember that non-specific antibody binding increases background. To prevent this, do not use too much primary or secondary antibody.
4. Detection reagents: Do not use excessive detection reagents. If the concentration is too high or not diluted correctly, it will result in a high background. Also don't overexpose, if necessary, optimize when you should add stop solution
PVC leather
Pvc Leather,Pvc Artificial Leather,Pvc Leather For Sofa,Pvc Leather For Car Seat
DONGGUAN SHENGYUAN INDUSTRIAL CO,.LTD. , https://www.elementpuleather.com