Method Preparation of the bacterial tablets: Staphylococcus aureus (ATCC6538), Escherichia coli (8099), Bacillus subtilis var. spores (ATCC9372) were diluted to a certain concentration of bacterial suspension with 0.03 mol/L phosphate buffer (PBS). Using a micro-sampler, take 20Ll of the suspension and dye it on a 10mm×10mm square glass piece and spread it evenly. Place two pieces in a sterile dish and dry at 37 °C for 20 minutes.
Sterilization test: The disinfection cabinet is divided into an ozone chamber and a far infrared chamber. The far infrared chamber is divided into upper and lower layers. During the test, the food (drinking) with water soaked (not dripping) is placed in the disinfection cabinet to the highest load (full load), and the plate for placing the bacteria is placed in the cabinet. 10cm at the door (outer) and the back wall (inside) (closed in the control group), the ozone button and the far infrared button are activated at the same time: the disinfection program stops and automatically switches to the protection state. When the temperature in the cabinet drops to room temperature, remove the bacteria. One piece of the bacteria is placed in a 5 ml PBS tube and shaken for 1 min to wash the bacteria on the bacteria tablets. Take 1 ml of the liquid or its dilution to inoculate the sterile plate, 37 Incubate for 48 h at ° C and calculate the kill rate.
Hepatitis B surface antigen destruction test: Hepatitis B surface antigen (HBsAg) purified at a concentration of 100 ug/ml was applied to a square glass piece (20 Ll/piece) having a diameter of 10 mm × 10 mm and spread uniformly, and dried at 37 ° C. After the disinfection cabinet (test process and HBsAg antigen tablet placement and sterilization test). (The positive control was placed outside the cabinet at room temperature) After disinfection, the HBsAg tablets were placed in a PBS tube containing 1 ml of 10% calf serum and shaken for 1 min to wash the HBsAg on the bacteria tablets. The ELZSA two-step method (reagent sensitivity was 1 ng/ Ml) Detection of HBsAg antigenicity. Using 10% fetal bovine serum PBS as a negative control, the test results showed that the ratio of the OD value (S) of the test group to the negative control OD value (N) (S/N) < 2.1 indicates that the antigenicity of HBsAg has been destroyed.
Test the temperature inside the cabinet: Place the probes of the temperature measuring instrument on the upper and lower layers of the far infrared chamber (the position is the same as the position of the sterilization test bacteria), close the cabinet door, turn on the power, and follow the disinfection procedure. The temperature of each point was recorded every 3 minutes.
Results Sterilization effect: The experiment showed that 114.8mg/m3 ozone acted on Staphylococcus aureus for 20min, ozone and far infrared were both applied for more than 20min, and the number of coliform bacteria in the control group was 1.200000cfu/tablet, Staphylococcus aureus 890000cfu/tablet, Bacillus subtilis The black variant spores were 525,000 cfu/tablet and the results were the average of three trials. Destruction of HBsAg antigenicity: The experiment showed that the ozone chamber could not destroy HBsAg antigenicity by ozone treatment at 114.48 mg/m3 for 20 min; while the far infrared chamber was used for one cycle, the HBsAg assay was negative.
Temperature inside the cabinet: After measuring, the temperature of the far infrared chamber rises faster with the inner point inside the cabinet, and the maintenance time is also long. The temperature is above 40°C for 40min, the lower point is 30min, the upper and outer points are 27min, and the lower outer point is Maintaining 20min.3 Discussion Since the introduction of the utensil disinfection cabinet in 1998, it has been developed from the electric food disinfection cabinet to the ozone utensil disinfection cabinet, the ultraviolet food disinfection cabinet and the electric heating and ozone combination utensil disinfection cabinet, ZTD-88 food. The disinfection cabinet is a combination of far infrared high temperature and ozone, and has a cleaning and disinfecting function. The results showed that the killing rate of Staphylococcus aureus was >98.00% in the 36L ozone chamber with 114.48mg/m3 for 20min; the killing rate for E. coli was >99.90%; the killing rate of Bacillus subtilis black variety was <40.00%, could not Destruction of HBsAg antigenicity. The killing rate of Escherichia coli and Staphylococcus aureus in the far infrared chamber is 100%. The killing rate of the black buds of the subtilis is different due to the high temperature (120-160 °C), and the killing rate is different. The point kill rate is 100%, and the upper and lower sides are >99.00%, which can completely destroy the antigenicity of HBsAg on the square glass piece.
The utensils disinfection cabinet, the ozone layer have good properties for the cleaning of the utensils, and the far-infrared high temperature disinfection of the utensils, and provide an ideal disinfection measure for the tableware hygiene in the household.
Descriptions for the series products:
1. The process of all the boards and walls of the series products are completed in one time by large machining center which can ensure the precision of all stations and the equipment operates more steadily.
2. The paper advance mechanism of facial tissue: We can ensure the paper feeding is in high speed and steady whether for thick paper or thin paper for the application of high speed dedicated Feeding Head.
3. Elevating stage of facial tissue: In line with the high speed FeiDa, We adopt high strength turbine reducer which can ensure the stability of accelerating instantly.
4. The paper supporter of facial tissue: We adopt the unique designation of E-type double gap which can push the produced paper directly with hand fork lifter, shorten the time of stacking paper and improve efficiency.
5. Body paper system: We adopt servo motor control system which have features of high sensitivity, high speed and can ensure the body paper output smooth, no jam.
6. Adsorption system of body paper: We adopt the inverter control which cooperates with the solenoid valve`s speedy switch to ensure the body paper is adsorbed completely and the operation is simple.
7. Glue wheel: The glue wheel is made by steel with thickening electroplating and the surface is treated as fine grinding. The amount of glue can be adjusted to 8g/㎡ to ensure the glue is even for thick paper and thin paper.
8. Supplement of glue: We adopt automatic level control to ensure the supplement of glue is automatic and the production is continuous.
9. Paper separation: We adopt the disengaging pawl to its high speed movements and make the paper separate from the glue wheel effectively after being daubed glue.
10. Fit orientation: We adopt the prelocalization and both sides have their own side board to ensure the high speed fit precision.
11. Driving system: We adopt synchronous belt drive imported from the United States to ensure the transmission is accurate, steady and has low noise.
12. Operating system: The equipment takes the man-machine touch screen as a operation center which is contacted with PLC, so that kinds of information, such as warning, detective hitch, abnormal condition and operating speed can be displayed on the screen, it`s absolutely clear. This can also detect maintenance log book, logging and look over in real-time, dispose timely which can truly reflects the efficiency.
13. Electric system: We adopt domestic and international famous brand, design in accordance with the European CE standard, manufacture meticulously to ensure the overall unite is stable, has high efficiency and low hitch.
14. If the machine arrangement is changed without prior notice.
Glue Laminator Machine, Gluing Laminator Machine, Fully Automatic Glue Laminator Machine
Anhui Innovo Bochen Machinery Manufacturing Co., Ltd. , https://www.innovomachines.com