**Detection Range:** 0.7 mIU/mL to 20 mIU/mL
**Purpose of Use:** This kit is designed for the quantitative determination of Glucose-6-Phosphate Dehydrogenase (G6PD) in human serum, plasma, and other related biological fluids. It provides a reliable and accurate method for assessing G6PD levels, which is essential for diagnosing conditions such as G6PD deficiency.
**Experimental Principle:** The assay is based on the double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) technique. A microplate is pre-coated with purified anti-G6PD monoclonal antibodies to serve as the solid-phase capture reagent. After adding the sample, G6PD binds to the immobilized antibody. An HRP-conjugated secondary antibody is then introduced, forming a complex of antibody-antigen-enzyme-labeled antibody. Following a thorough wash step, TMB substrate is added, and the reaction is catalyzed by HRP, resulting in a color change from blue to yellow. The intensity of the color is directly proportional to the concentration of G6PD in the sample. The optical density (OD) is measured at 450 nm using a microplate reader, and the G6PD concentration is calculated using a standard curve.
**Kit Composition:**
1. 30× Washing Solution – 20 mL × 1 bottle
2. Stop Solution – 6 mL × 1 bottle
3. Enzyme Standard Reagent – 6 mL × 1 bottle
4. Enzyme-Labeled Coating Plate – 12 wells × 8 strips
5. Sample Diluent – 6 mL × 1 bottle
6. TMB Color Reagent A – 6 mL × 1 bottle
7. TMB Color Reagent B – 6 mL × 1 bottle
8. Standard Product (40 mIU/mL) – 0.5 mL × 1 bottle
9. Standard Dilutions – 1.5 mL × 1 bottle
10. Instructions – 1 booklet
11. Sealing Film – 2 sheets
12. Aluminum Seal Bag – 1 bag
This kit is ideal for use in clinical laboratories and research settings where precise measurement of G6PD is required. It ensures high sensitivity, specificity, and reproducibility, making it a valuable tool for diagnostic and investigative purposes. Proper storage and handling are recommended to maintain the integrity and performance of the reagents.
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