Anaerobic incubators are essential tools in microbiology laboratories, designed to create and maintain an oxygen-free environment for the cultivation of anaerobic bacteria. These devices play a crucial role in research, diagnostics, and biotechnology, allowing scientists to study organisms that cannot survive in the presence of oxygen. Shanghai Xinzhuang Instrument Co., Ltd. is a leading manufacturer specializing in the development, production, and sales of high-quality anaerobic incubators. With a strong focus on technological innovation and customer satisfaction, the company continues to deliver advanced solutions that meet the evolving needs of the scientific community.
**Applications**
An anaerobic incubator is specifically designed for cultivating microorganisms in environments devoid of oxygen. It enables researchers to safely handle and grow even the most sensitive anaerobic organisms without exposing them to atmospheric oxygen, which can be harmful or fatal. This makes it an indispensable tool in medical research, pharmaceutical development, and environmental studies.
**Structure and Function**
The device typically consists of several key components: a constant-temperature cultivation chamber, an anaerobic operation room, a sample transfer chamber, a gas supply system, and an electrical control panel. The design allows for seamless operation, with features such as a dual-hand operation port, an efficient gas exchange system, and automated monitoring functions. This ensures a stable and safe anaerobic environment throughout the entire process.
**Key Features**
- Compact and space-efficient design, ideal for crowded labs.
- Easy-to-use interface with automated controls for temperature, humidity, and gas flow.
- Dual-hand operation eliminates the need for cumbersome gloves, improving user comfort.
- Ergonomic design reduces the risk of occupational injuries during long sessions.
- Complete standard components for hassle-free setup and use.
**Installation Guidelines**
When setting up the anaerobic incubator, ensure it is placed in a stable, temperature-controlled area away from direct sunlight and heat sources. Proper installation of gas cylinders and pressure regulators is essential, along with regular checks for air leaks. The gas lines should be securely connected and sealed to prevent contamination or loss of the anaerobic environment.
**Operation Instructions**
To establish an anaerobic environment in the operating chamber:
1. Place necessary equipment and two nontoxic plastic bags inside the chamber.
2. Adjust the pressure of the mixed gas and nitrogen cylinders to 0.1 MPa.
3. Power on the device and add palladium particles and an anaerobic indicator.
4. Perform multiple nitrogen flushes to remove oxygen from the chamber.
5. Replace the gas with a mixture of hydrogen, carbon dioxide, and nitrogen to achieve the desired anaerobic conditions.
6. Monitor the oxygen levels using the anaerobic indicator and ensure all systems are functioning properly.
**Inoculation and Culture**
After confirming the anaerobic state, samples can be introduced through the transfer chamber. The process involves vacuuming and replacing the gas multiple times to ensure complete removal of oxygen. Once the environment is stable, cultures can be maintained for extended periods with periodic replenishment of gases and replacement of oxygen scavengers.
**Safety and Maintenance Tips**
- Always check for air leaks before and after each use.
- Avoid exposing the device to extreme temperature fluctuations.
- Regularly inspect and replace filters, desiccants, and catalysts.
- In case of power failure, the anaerobic environment can last up to 12 hours.
- Follow proper procedures when changing gas cylinders to prevent oxygen ingress.
Shanghai Xinzhuang Instrument Co., Ltd. is committed to providing reliable, innovative, and user-friendly anaerobic incubators that support cutting-edge research and laboratory practices. For more information or to place an order, feel free to contact us directly.
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